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. 2017 Dec 21;12(12):e0188179. doi: 10.1371/journal.pone.0188179

Fig 2. Comparison of the antifungal activities of the wild-type and surfactin-less mutants B. subtilis subsp. krictiensis ATCC55079 strains against F. oxysporum.

Fig 2

(A) Comparison of the antifungal activities of authentic iturin (top) and surfactin (bottom) against various fungi. Two-fold serially dilutions of iturin and surfactin were loaded into PDA plates containing M. grisea (concentration range, 1.56–12.5 μg/ml; left), F. oxysporum (6.25–50 μg/mL; right), and a Sabouraud plate containing T. mentagrophytes (3.12–25 μg/mL; center). (B) Comparison of the antifungal activities of the wild-type, mutant-22, and mutant-31strains. Antifungal activities against F. oxysporum were examined using culture broth (250 μL) from each strain. Data are expressed as means ± SD for separate experiments performed in sextuplicate.