| Bacterial strains |
| Escherichia coli DH5α |
F- Φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rk- mk+) supE44 relA1deoRΔ(lacZYA-argF) U169 |
Promega, Madison, WI |
| Escherichia coli HB101 |
F-
hsdS20 (rB- mB-) recA13 ara-14 proA2 lacY1 galK2rpsL20 (Strr) xyl-5 mtl-1 supE44 thi-1 leuB6 |
Promega, Madison, WI |
| Bacillus subtilis 168 |
trpC2 |
[26] |
| Bacillus subtilis subsp. krictiensis ATCC55079 |
Wild-type |
[28] |
| Fungi |
| Fusarium oxysporum
|
Wild-type |
This study |
| Magnaporthe grisea
|
Wild-type |
This study |
| Trichophyton mentagrophytes
|
Wild-type |
This study |
| Plasmids |
| pTZ18R |
Ampr plasmid carrying the T7 g10 promoter |
[31] |
| pLAFR1 |
pRK290 containing the cos site |
[32] |
| pLAFR3 |
pLAFR1 containing an HaeII fragment of pUC8 |
[32] |
| pIC333 |
Mini-Tn10 delivery vector |
[33] |
| pJJ5, pJJ71, pJJ121, pJJ815 |
Genomic library clones constructed using cosmid vector pLAFR3 |
This study |
| pJJ121E2 |
16.4-kb EcoRI-digested pJJ121 fragment inserted into pTZ18R |
This study |
| pJJ121E3 |
4.8-kb EcoRI-digested pJJ121 fragment inserted into pTZ18R |
This study |
| pJJ815E4 |
3.7-kb EcoRI-digested pJJ815 fragment |
This study |
| pJJ121E2-2 |
7.9-kb EcoRI and SalI-digested pJJ121E2 fragment inserted into pBC KS(+) |
This study |
| pJJ121E2-1 |
BamHI and XbaI-digested pIC333 fragment inserted into the ClaI site of pJJ121E2-2 |
This study |
