Skip to main content
. 2017 Dec 21;12(12):e0189072. doi: 10.1371/journal.pone.0189072

Fig 1. 13C labelling of intermediates in central carbon metabolism in T. vaginalis and T. foetus.

Fig 1

T. vaginalis and T. foetus were grown at 37°C in MDM containing 100% D-[U-13C6] glucose as the only carbohydrate carbon source. Cell extracts were prepared after 2 h, 6 h and 20 h in culture and analysed by LC-MS. (A) Map of glycolysis and central carbon metabolism in T. vaginalis and T. foetus. Reactions found only in T. foetus are shown by dashed lines. T. vaginalis uses pyrophosphate (ppi) dependent enzymes for the steps indicated [32]. Metabolites detected in the 13C labelling experiment are boxed. (B) Map of pyruvate metabolism in the hydrogenosome in T. vaginalis. Dashed lines indicate the NADH dehydrogenase complex I. Metabolites detected in the 13C labelling experiment are boxed. (C) 13C labelling of metabolites in T. vaginalis and T. foetus. Graphs show the percentage of isotopologues with different numbers of 13C carbon atoms.