Fig 5. Comparison of total levels of intermediates in nucleotide and methionine metabolism from extracts of ¹³C labelled cells.

Total metabolite levels represent the sum of the peak intensities of all isotopologues of metabolites detected in extracts of ¹³C labelled cells. Graphs show the log2 fold change in total peak intensities after 2, 6 and 20 h growth. Results represent the mean of 3 independent cultures ± SD. Dashed line indicates the threshold for a definite difference between the species (3-fold or log2 1.6-fold change in metabolite level). Positive values represent a relative increase in T. vaginalis, negative values a relative decrease. Ribose-5P, Ribose-5-phosphate; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; MTA, 5-methylthioadenosine; OPS, O-phosphoserine; SMC, S-methylcysteine. * P ≤ 0.001 (Paired T-Test, assuming equal variance).