Table 1. Metabolites from cell extracts showing more than 3-fold difference between species.
| Metabolite | Formula | T. vaginalis Mean PI | T. foetus Mean PI | Tvag/Tfoe | Pathway |
|---|---|---|---|---|---|
| Malonate | C3H4O4 | 1.15E+05 | 3.04E+04 | 3.79 | Pyrimidine/ β-alanine |
| 2-Hydroxybutanoate | C4H8O3 | 3.97E+06 | 5.69E+04 | 69.84 | Propanoate |
| Uracil | C4H4N2O2 | 6.29E+05 | 2.52E+06 | 0.25 | Pyrimidine |
| Creatinine | C4H7N3O | 1.48E+06 | 2.91E+05 | 5.07 | Arginine and proline |
| Succinate | C4H6O4 | 3.48E+06 | 6.41E+07 | 0.05 | Glucose metabolism |
| 5-Oxoproline | C5H7NO3 | 6.30E+07 | 1.45E+07 | 4.35 | Arginine and proline |
| L-Pipecolate | C6H11NO2 | 7.63E+07 | 8.90E+06 | 8.57 | Lysine |
| Creatine | C4H9N3O2 | 8.18E+05 | 1.18E+05 | 6.93 | Arginine and Proline |
| HICA | C6H12O3 | 1.82E+07 | 2.49E+06 | 7.3 | Leucine |
| L-Ornithine | C5H12N2O2 | 1.45E+05 | 4.26E+05 | 0.34 | Arginine and Proline |
| Malate | C4H6O5 | 8.58E+06 | 2.08E+06 | 4.12 | Glucose metabolism |
| SMC | C4H9NO2S | 1.15E+06 | 5.43E+03 | 212.62 | Unknown |
| Hypoxanthine | C5H4N4O | 6.47E+06 | 9.57E+05 | 6.76 | Purine |
| Histidinal | C6H9N3O | 5.05E+05 | 4.00E+04 | 12.63 | Histidine |
| 4-guanidinobutanoate | C5H11N3O2 | 2.49E+05 | 4.96E+04 | 5.03 | Arginine and Proline |
| 2-ketoglutarate | C5H6O5 | 5.42E+05 | 3.75E+06 | 0.14 | Glutamate |
| L-Lysine | C6H14N2O2 | 1.47E+06 | 6.02E+06 | 0.24 | Lysine |
| Imidazolone lactate | C6H8N2O3 | 1.30E+06 | 1.38E+04 | 94.1 | Histidine |
| N6-Methyl-L-lysine | C7H16N2O2 | 2.12E+04 | 2.72E+03 | 7.79 | Lysine |
| L-Carnitine | C7H15NO3 | 3.03E+04 | 9.96E+03 | 3.04 | Lysine/ acylcarnitine |
| Phenyllactate | C9H10O3 | 1.02E+07 | 9.39E+05 | 10.9 | Phenylalanine |
| N(pi)-Methyl-L-histidine | C7H11N3O2 | 1.82E+05 | 4.50E+04 | 4.04 | Histidine |
| Indole-3-acetate | C10H9NO2 | 7.57E+03 | 1.98E+03 | 3.82 | Tryptophan |
| L-Citrulline | C6H13N3O3 | 9.40E+05 | 5.32E+06 | 0.18 | Arginine |
| D-Glucosamine | C6H13NO5 | 5.15E+03 | 1.54E+03 | 3.34 | Amino / Nucleotide sugar |
| N6-Acetyl-L-lysine | C8H16N2O3 | 2.62E+05 | 9.68E+05 | 0.27 | Lysine |
| Carboxyspermidine | C8H19N3O2 | 1.00E+05 | 1.57E+04 | 6.41 | Arginine and Proline |
| D-Gluconic acid | C6H12O7 | 1.21E+07 | 1.37E+05 | 88.35 | Pentose Phosphate |
| Indolepyruvate | C11H9NO3 | 3.88E+03 | 1.13E+03 | 3.42 | Tryptophan |
| O-Acetylcarnitine | C9H17NO4 | 1.11E+05 | 1.61E+04 | 6.88 | Acylcarnitine |
| Pantothenate | C9H17NO5 | 4.51E+05 | 1.33E+05 | 3.4 | Pantothenate |
| L-Cystathionine | C7H14N2O4S | 1.19E+04 | 3.24E+05 | 0.04 | Methionine |
| L-Cystine | C6H12N2O4S2 | 7.27E+03 | 1.01E+05 | 0.07 | Cysteine |
| Cysteine: Homocysteine disulfide | C7H14N2O4S2 | 0.00E+00 | 1.06E+05 | 0 | Unknown |
| Thiamin | C12H16N4OS | 2.92E+05 | 1.64E+06 | 0.18 | Thiamine |
| Inosine | C10H12N4O5 | 4.27E+05 | 8.26E+04 | 5.17 | Purine |
| Guanosine | C10H13N5O5 | 2.67E+03 | 2.30E+04 | 0.12 | Purine |
| N-Acetyl-D-Glucosamine 6-phosphate | C8H16NO9P | 9.16E+04 | 3.06E+04 | 3 | Amino / Nucleotide sugar |
| N-Acetylneuraminate | C11H19NO9 | 8.23E+03 | 5.49E+04 | 0.15 | Amino / Nucleotide sugar |
| Sucrose | C12H22O11 | 2.27E+05 | 3.61E+04 | 6.28 | Carbohydrate |
| IMP | C10H13N4O8P | 3.88E+04 | 7.81E+03 | 4.97 | Purine |
| SAH | C14H20N6O5S | 2.78E+04 | 1.53E+05 | 0.18 | Methionine |
| UDP-N-acetyl-D-glucosamine | C17H27N3O17P2 | 2.49E+06 | 6.22E+05 | 3.99 | Amino / Nucleotide sugar |
| NADP+ | C21H28N7O17P3 | 1.06E+05 | 4.83E+05 | 0.22 | Nicotinate and Nicotinamide |
The table shows mean peak intensities (PI) from LCMS analysis of T. vaginalis and T. foetus cell extracts. Numbers represent the mean of at least four extracts from independent cultures. Tvag/Tfoe indicates the ratio of the PI values. P-values ≤0.005 (Paired T-Test, assuming equal variance). Except for malonate, 4-guanidinobutanoate and indole pyruvate where P ≤ 0.05. Full details including individual standard deviation and P values are shown in S2 Table.
HICA, 2-hydroxyisocaproate; SAH, S-adenosyl-L-homocysteine; SMC, S-methylcysteine.