Table 2. Metabolites taken up or exported into media during growth of T. vaginalis and T. foetus.
| Metabolite | Formula | MDMc Mean PI | TvagSPT Mean PI | TfoeSPT Mean PI | TvagSPT/ MDMc | TfoeSPT/ MDMc | TvagSPT/ TfoeSPT |
|---|---|---|---|---|---|---|---|
| Putrescine | C4H12N2 | 4.34E+03 | 7.56E+03 | 2.08E+04 | 1.74 | 4.79 | 0.36 |
| 2-Hydroxybutanoate | C4H8O3 | 1.55E+05 | 4.94E+06 | 1.64E+05 | 31.85 | 1.06 | 30.15 |
| Succinate | C4H6O4 | 1.08E+07 | 1.10E+07 | 4.35E+07 | 1.02 | 4.03 | 0.25 |
| Nicotinamide | C6H6N2O | 1.24E+06 | 4.49E+03 | 1.12E+06 | 0.00 | 0.91 | 0.00 |
| L-Pipecolate | C6H11NO2 | 1.77E+06 | 1.87E+07 | 2.41E+06 | 10.53 | 1.36 | 7.74 |
| HICA | C6H12O3 | 2.10E+05 | 1.31E+07 | 2.87E+05 | 62.44 | 1.37 | 45.59 |
| SMC | C4H9NO2S | 6.65E+03 | 2.64E+05 | 6.21E+03 | 39.70 | 0.93 | 42.57 |
| Hypoxanthine | C5H4N4O | 7.72E+05 | 8.10E+06 | 1.01E+06 | 10.49 | 1.30 | 8.04 |
| Ethanolamine phosphate | C2H8NO4P | 7.67E+03 | 1.05E+04 | 3.71E+04 | 1.37 | 4.84 | 0.28 |
| Spermidine | C7H19N3 | 2.92E+06 | 1.90E+05 | 2.66E+05 | 0.07 | 0.09 | 0.71 |
| Guanine | C5H5N5O | 1.06E+05 | 1.54E+04 | 1.29E+05 | 0.15 | 1.21 | 0.12 |
| Phenyllactate | C9H10O3 | 2.59E+05 | 7.41E+06 | 5.54E+05 | 28.57 | 2.14 | 13.36 |
| O-Phosphoserine | C3H8NO6P | 3.32E+04 | 3.80E+03 | 3.55E+04 | 0.11 | 1.07 | 0.11 |
| Indolelactate | C11H11NO3 | 1.96E+04 | 1.11E+06 | 1.54E+05 | 56.53 | 7.86 | 7.19 |
| Adenosine | C10H13N5O4 | 1.04E+07 | 5.53E+04 | 1.04E+07 | 0.01 | 1.00 | 0.01 |
| Inosine | C10H12N4O5 | 3.29E+05 | 9.54E+04 | 3.86E+05 | 0.29 | 1.17 | 0.25 |
| Guanosine | C10H13N5O5 | 4.03E+05 | 1.54E+04 | 4.98E+05 | 0.04 | 1.24 | 0.03 |
| MTA | C11H15N5O3S | 1.54E+05 | 6.81E+02 | 1.36E+05 | 0.00 | 0.89 | 0.00 |
| GDP | C10H15N5O11P2 | 0.00E+00 | 7.30E+04 | 1.48E+04 | INF | INF | 4.95 |
The table shows mean peak intensities for samples from complete or uninfected MDM media (MDMc), spent media (culture supernatents) from T. vaginalis cultures (TvagSPT) and spent media from T. foetus cultures (TfoeSPT). Values represent mean from at least 4 independent cultures. Ratios of spent media and complete media (TvagSPT/MDMc) and (TfoeSPT/MDMc) indicate whether a metabolite is taken up or released from cells. Differences between species are shown by the ratio of spent media (TvagSPT/TfoeSPT). P ≤ 0.005 (Paired T-Test, assuming equal variance. Except for putrescine and spermidine, P ≤ 0.05. Full details including individual standard deviation and P values are shown in S3 Table where metabolites are also categorized according to metabolic pathways.
HICA, 2-hydroxyisocaproate, MTA, 5-methylthioadenosine; SMC, S-methylcysteine.