Table 5. Kinetic analysis of SMC synthase and CS synthase activities of T. vaginalis CS.
Enzyme activity | Substrate | Vmax (nmol/min/mg) | Km (mM) | kcat(S-1) | kcat/Km (M-1s-1) |
---|---|---|---|---|---|
SMC synthase | CH3SNa (with 10 mM OPS) | 7.3 ± 2.4 | 4.3 ± 1.6 | 4.1 | 1.0 x 103 |
OPS (with 3 mM CH3SNa) | 4.4 ± 1.4 | 4.6 ± 1.9 | 2.5 | 5.5 x 102 | |
Cysteine synthase | Na2S (with 50 mM OPS) | 130 ± 3.6 | 0.4 ± 0.8 | 0.2 | 2.3 x 104 |
OPS (with 3 mM Na2S) | 20.4 ± 4.2 | 18.2 ± 0.8 | 11.5 | 6.4 x 102 |