Skip to main content
. 2017 Dec 21;12(12):e0189072. doi: 10.1371/journal.pone.0189072

Table 5. Kinetic analysis of SMC synthase and CS synthase activities of T. vaginalis CS.

Enzyme activity Substrate Vmax (nmol/min/mg) Km (mM) kcat(S-1) kcat/Km (M-1s-1)
SMC synthase CH3SNa (with 10 mM OPS) 7.3 ± 2.4 4.3 ± 1.6 4.1 1.0 x 103
OPS (with 3 mM CH3SNa) 4.4 ± 1.4 4.6 ± 1.9 2.5 5.5 x 102
Cysteine synthase Na2S (with 50 mM OPS) 130 ± 3.6 0.4 ± 0.8 0.2 2.3 x 104
OPS (with 3 mM Na2S) 20.4 ± 4.2 18.2 ± 0.8 11.5 6.4 x 102