Table 1. Antibodies employed for IF, IP and WBa.
Gene name | Catalog No. | Vendor | Application | Dilution Factor |
---|---|---|---|---|
Aco2 | 6922 | Cell Signaling | WB | 1000 |
a-actinin, sarcomeric | A7811 | Sigma | IF | 50 |
Atp5a | ab14748 | Abcam | WB | 1000 |
Bak | 3184 | Cell Signaling | WB | 1000 |
Bax | 2772 | Cell Signaling | WB | 1000 |
BrdU | MCA2060 | AbD Serotec | IF | 50 |
Cat | 14097 | Cell Signaling | WB | 1000 |
Complex I | ab109798 | Abcam | ELISA | 5 ug |
Cox4i1 | ab16056 | Abcam | WB | 1000 |
Cx43 | CBL171 | Sigma | WB | 1000 |
CytC | ab13575 | Abcam | WB | 1000 |
E2f1 | sc-193 | Santa Cruz | WB | 500 |
Erk1/2 | 9102 | Cell Signaling | WB | 1000 |
Erk1/2 Pi-Thr202/Tyr204 | 9101 | Cell Signaling | WB | 1000 |
Esrra/b | NBP1-47254 | Novus | WB | 1000 |
Esrrg | 27–388 | ProScience | WB | 1000 |
Gpx4 | Ab125066 | Abcam | WB | 1000 |
Gsta1 | sc-100546 | Santa Cruz | WB | 500 |
Lam | L9393 | Sigma | WB | 1000 |
Mdm2 | M8558 | Sigma | WB | 1000 |
Mef2a | ab32866 | Abcam | IF | 50 |
Myc | sc-764 | Santa Cruz | WB | 500 |
Ndufa8 | BS3336 | BioWorld | WB | 1000 |
normal rabbit IgG | 2729 | Cell Signaling | IP | 7.5 ug—1000 |
Npm1 | B0556 | Sigma | WB | 1000 |
Opa1 | ab42364 | Abcam | WB | 1000 |
p38 | 9112 | Cell Signaling | WB | 1000 |
p38 Pi-Thr180/Tyr182 | 9211 | Cell Signaling | WB | 1000 |
p53 | BML-SA293 | Enzo Life Sci. | IP WB | 1000 |
Pgc—1a | 3934–100 | BioVision | WB | 1000 |
Pink1 | 3929–100 | BioVision | WB | 1000 |
Pln | 8495 | Cell Signaling | WB | 1000 |
Ppara | PAB11321 | Abnova | WB | 1000 |
Sdhb | ab14714 | Abcam | WB | 1000 |
SERCA2 | 9580 | Cell Signaling | WB | 1000 |
Sirt3 | 5490 | Cell Signaling | WB | 1000 |
Sod2 | 611580 | Becton Dickinson | WB | 4000 |
Txnrd2 | ab16841 | Abcam | WB | 1000 |
ubiquitin | 13–1600 | Thermo Fisher | WB | 7.5 ug |
aIF, immunofluorescence microscopy. IP, immunoprecipitation. WB, Western blot.