Fig 4. Probability distributions of hydrodynamic radius obtained by DLS.

A) Distribution of protein radius for 0.2 mM BSA and distinct values of urea concentrations. Color bar represents urea concentration. Distributions indicate progressive protein unfolding with increasing urea concentration. B) Comparisons of hydrodynamic radius, obtained from fits based in Eq 6 and full Sader’s model (Fig 3B), with values from DLS distributions (Fig 4A).