Table 1. Physical parameters, plasma and arterial gas analysis parameters.
| Sham | UNx | IR | IR+UNx | |
|---|---|---|---|---|
| BW [g] (baseline) | 295 ± 11.5 | 305 ± 10.5 | 296 ± 8.4 | 305 ± 12.2 |
| Δ BW [g] | -4.0 ± 6.1 | 1.9 ± 4.3 | 3.8 ± 2.8 | 5.7 ± 2.9 |
| KW [g/100 g BW] | 0.33 ± 0.01 | 0.38 ± 0.02 | 0.43 ± 0.014 (LK) *** 0.38 ± 0.012 (RK) $ |
0.40 ± 0.01 * |
| Pcr [μmol/L] | 26 ± 1.9 | 41 ± 1.6 * | 39 ± 1.7 | 198 ± 42.6 ****, # |
| Purea [mmol/L] | 6 ± 0.2 | 8 ± 0.3 | 8 ± 0.3 | 25 ± 4.1 **** |
| PO2 [kPa] | 8.4 ± 0.6 | 9.2 ± 0.9 | 9.5 ± 0.6 | 11.0 ± 0.9 |
| Hb [mmol/L] | 7.3 ± 0.3 | 7.7 ± 0.1 | 8,2 ± 0.3 | 7.6 ± 0.1 |
| Hct [%] | 36 ± 1.4 | 38 ± 0.6 | 40 ± 1.6 | 37 ± 0.6 |
| SO2 [%] | 78 ± 4 | 80 ± 5 | 83 ± 3 | 88 ± 1 |
Values are presented as means ± SEM. Measurements are from the day of termination unless otherwise stated. Comparison of unpaired group means was performed using either two-way ANOVA followed by Bonferroni’s multiple comparisons post-test or Kruskal-Wallis test with Dunn's multiple comparisons post-test.
*, P < 0.05
***, P < 0.005
****, P < 0.0001 vs. sham
#, P < 0.05 vs. IR. Comparison of paired group means was performed by Wilcoxon signed rank test.
$, P < 0.01 vs. left kidney (LK).
BW, body weight; KW, kidney weight; Pcr, plasma creatinine; Purea, plasma urea; PO2, partial pressure of oxygen; Hb, hemoglobin; Hct: hematocrit; SO2, oxygen saturation; UNx, unilateral nephrectomy; IR, ischemia-reperfusion. Number of animals: sham, UNx and IR groups (n = 8), IR+UNx (n = 6).