Table 4. The binding fraction and sum of H+ binding (∑H+) of metabolite-cation complexes for non-mitochondrial muscle energy metabolism.
| Non-competitive# | Multiple Competitive Cation Binding | |||||
|---|---|---|---|---|---|---|
| Complex | pH = 6.0 | pH = 7.0 | pH = 6.0 | pH = 7.0 | Polynomial* | |
| Phosphagen | ||||||
| Pi-3 | 3.0581E-7 | 1.1323E-5 | A = -0.002923; B = 0.001429; C = -0.0002332; D = 1.271E-5 | |||
| Pi-H | 0.1190 | 0.4405 | A = 34.78; B = -16.31; C = 2.513; D = -0.1263 | |||
| Pi-H2 | 0.8490 | 0.3599 | 0.6690 | 0.2477 | A = -44.77; B = 21.37; C = -3.293; D = 0.1655 | |
| Pi-H3 | 6.5382E-5 | 2.4208E-6 | A = 0.02009; B = -0.008669; C = 0.00125; D = -6.014E-5 | |||
| Pi-Mg | 4.6090E-7 | 1.7065E-5 | A = -0.004405; B = 0.002154; C = -0.0003515; D = 1.915E-5 | |||
| Pi-HMg | 4.5041E-3 | 1.6676E-2 | A = 1.317; B = -0.6174; C = 0.09513; D = -0.00478 | |||
| Pi-H2Mg | 2.0119E-3 | 7.4491E-4 | A = -0.1346; B = 0.06428; C = -0.009903; D = 0.0004976 | |||
| Pi-K | 1.4610E-7 | 5.4092E-6 | A = -0.001396; B = 0.0006827; C = -0.0001114; D = 6.071E-6 | |||
| Pi-HK | 4.5148E-2 | 0.1672 | A = 13.2; B = -6.189; C = 0.9535; D = -0.04792 | |||
| Pi-H2K | 0.1243 | 4.6040E-2 | A = -8.32; B = 3.973; C = -0.6121; D = 0.03076 | |||
| Pi-HK2 | 1.2411E-2 | 4.5953E-2 | A = 3.629; B = -1.701; C = 0.2621; D = -0.01317 | |||
| Pi-K2 | 9.2001E-8 | 3.4065E-6 | A = -0.0008794; B = 0.0004299; C = -7.017E-5; D = 3.823E-6 | |||
| Pi-Na | 9.5840E-8 | 3.5485E-6 | A = -0.000916; B = 0.0004479; C = -7.309E-5; D = 3.982E-6 | |||
| Pi-HNa | 7.2702E-3 | 2.6918E-2 | A = 2.126; B = -0.9966; C = 0.1535; D = -0.007716 | |||
| Pi-NaH2 | 1.5543E-2 | 5.7550E-3 | A = -1.04; B = 0.4966; C = -0.07651; D = 0.003845 | |||
| Pi-Na2 | 1.6130E-8 | 5.9722E-7 | A = -0.0001542; B = 7.538E-5; C = -1.23E-5; D = 6.702E-7 | |||
| Pi-Na2H | 6.7242E-4 | 2.4896E-3 | A = 0.1966; B = -0.09217; C = 0.0142; D = -0.0007136 | |||
| ∑H (Fraction) | 1.8111 | 1.3002 | A = -53.21; B = 25.89; C = -3.988; D = 0.2004 | |||
| IMP-1 | 0.3109 | 0.8016 | A = 43.5; B = -21.53; C = 3.495; D = -0.1843 | |||
| IMP-H | 0.6863 | 0.1795 | 0.6802 | 0.1754 | A = -43.75; B = 22.15; C = -3.595; D = 0.1896 | |
| IMP-H2 | 1.3571E-5 | 3.4991E-7 | A = 0.00513; B = -0.002233; C = 0.0003244; D = -1.573E-5 | |||
| IMP-H3 | 3.4089E-11 | 8.7894E-14 | A = 2.853E-8; B = -1.276E-8; C = 1.901E-9; D = -9.441E-11 | |||
| IMP-Mg | 3.4089E-11 | 8.7894E-14 | A = 1.249; B = -0.6183; C = 0.1004; D = -0.005293 | |||
| ∑H (Fraction) | 0.6802 | 0.1754 | A = -43.74; B = 22.15; C = -3.595; D = 0.1896 | |||
| AMP-1 | 0.2971 | 0.5182 | A = -2.627; B = 0.1279; C = 0.1438; D = -0.01398 | |||
| AMP-H | 0.6131 | 0.1368 | 0.4708 | 0.0821 | A = 4.207; B = 0.4092; C = -0.3479; D = 0.02933 | |
| AMP-H2 | 0.4708 | 0.0821 | A = 1.446; B = -0.6357; C = 0.09325; D = -0.004562 | |||
| AMP-Mg | 2.9708E-2 | 2.9016E-2 | A = -0.1471; B = 0.007162; C = 0.008052; D = -0.0007831 | |||
| AMP-K | 0.1787 | 0.3116 | A = -1.58; B = 0.07694; C = 0.08648; D = -0.008411 | |||
| AMP-Na | 3.3804E-2 | 5.8963E-2 | A = -0.2989; B = 0.01456; C = 0.01636; D = -0.001591 | |||
| ∑H (Fraction) | 0.4768 | 0.0822 | A = 7.098; B = -0.8623; C = -0.1614; D = 0.02021 | |||
| NH3-1 | 5.4924E-4 | 5.4653E-4 | A = -0.8459; B = 0.4163; C = -0.06853; D = 0.003775 | |||
| NH3-H | 0.9995 | 0.9945 | 0.9994 | 0.9945 | A = 1.847; B = -0.4167; C = 0.06859; D = -0.003779 | |
| NH3-Mg | 4.6549E-7 | 4.6320E-6 | A = -0.0007169; B = 0.0003529; C = -5.808E-5; D = 3.2E-6 | |||
| ∑H (Fraction) | 0.9994 | 0.9945 | A = 1.847; B = -0.4167; C = 0.06859; D = -0.003779 | |||
| ADP-4 | 0.1691 | 0.2713 | A = -4.365; B = 1.483; C = -0.1452; D = 0.003993 | |||
| ADP-H | 0.7058 | 0.1935 | 0.4057 | 6.5077E-2 | A = 14.14; B = -4.321; C = 0.3905; D = -0.008627 | |
| ADP-H2 | 3.0074E-3 | 4.8242E-5 | A = 1.546; B = -0.6814; C = 0.1001; D = -0.004908 | |||
| ADP-H3 | 1.4729E-7 | 2.3628E-10 | A = 0.0001375; B = -6.164E-5; C = 9.211E-6; D = -4.586E-7 | |||
| ADP-Mg | 0.1723 | 0.2764 | A = -4.447; B = 1.511; C = -0.148; D = 0.004068 | |||
| ADP-HMg | 9.4670E-3 | 1.5191E-3 | A = 0.3301; B = -0.1009; C = 0.009114; D = -0.0002014 | |||
| ADP-Mg2 | 4.0224E-3 | 6.453E-3 | A = -0.1038; B = 0.03528; C = -0.003455; D = 9.497E-5 | |||
| ADP-K | 0.2029 | 0.3255 | A = -5.237; B = 1.78; C = -0.1743; D = 0.004792 | |||
| ADP-Na | 3.3441E-2 | 5.3644E-2 | A = -0.863; B = 0.2933; C = -0.02872; D = 0.0007896 | |||
| ∑H (Fraction) | 0.4212 | 6.6669E-2 | A = 17.56; B = -5.785; C = 0.5999; D = -0.01865 | |||
| ATP-4 | 6.9979E-2 | 8.9269E-2 | A = -2.695; B = 1.14; C = -0.1562; D = 0.007161 | |||
| ATP-H | 0.7512 | 0.2319 | 0.2113 | 2.6959E-2 | A = 25.81; B = -10.52; C = 1.436; D = -0.0656 | |
| ATP-H2 | 2.0652E-3 | 2.6345E-5 | A = 1.231; B = -0.545; C = 0.0805; D = -0.003964 | |||
| ATP-H3 | 1.6405E-7 | 2.0927E-10 | A = 0.0001619; B = -7.273E-5; C = 1.088E-5; D = -5.427E-7 | |||
| ATP-H4 | 1.6405E-12 | 2.0927E-16 | A = 2.034E-9; B = -9.184E-10; C = 1.382E-10; D = -6.924E-12 | |||
| ATP-Mg | 0.5286 | 0.6743 | A = -20.36; B = 8.611; C = -1.18; D = 0.05409 | |||
| ATP-HMg | 2.6492E-2 | 3.3379E-3 | A = 3.235; B = -1.319; C = 0.18; D = -0.008223 | |||
| ATP-Mg2 | 1.5895E-2 | 2.0203E-2 | A = -0.6122; B = 0.259; C = -0.03547; D = 0.001627 | |||
| ATP-K | 0.12442 | 0.1584 | A = -4.783; B = 2.024; C = -0.2772; D = 0.01271 | |||
| ATP-Na | 2.1432E-2 | 2.7340E-2 | A = -0.8254; B = 0.3492; C = -0.04783; D = 0.002193 | |||
| ∑H (Fraction) | 0.2420 | 0.0304 | A = 31.5; B = -12.93; C = 1.777; D = -0.08175 | |||
| CrP-3 | 3.8454E-9 | 3.9391E-8 | A = -6.198E-6; B = 3.049E-6; C = -5.016E-7; D = 2.762E-8 | |||
| CrP-H | 0.9693 | 0.9968 | 0.7673 | 0.7860 | A = -3.893; B = 2.003; C = -0.2867; D = 0.01372 | |
| CrP-H2 | 2.4263E-2 | 2.4854E-3 | A = 5.447; B = -2.33; C = 0.3335; D = -0.01596 | |||
| CrP-H3 | 1.2160E-5 | 1.2457E-7 | A = 0.008278; B = -0.003682; C = 0.0005461; D = -2.7E-5 | |||
| CrP-H4 | 1.2160E-9 | 1.2465E-12 | A = 1.267E-6; B = -5.698E-7; C = 8.539E-8; D = -4.263E-9 | |||
| CrP-HMg | 1.8327E-2 | 1.8774E-2 | A = -0.09299; B = 0.04784; C = -0.006848; D = 0.0003278 | |||
| CrP-K | 2.5358E-9 | 2.5976E-8 | A = -4.088E-6; B = 2.011E-6; C = -3.308E-7; D = 1.821E-8 | |||
| CrP-HK | 0.1880 | 0.1926 | A = -0.9539; B = 0.4907; C = -0.07024; D = 0.003362 | |||
| CrP-H2K | 2.158E-3 | 2.2211E-4 | A = 0.4846; B = -0.2073; C = 0.02967; D = -0.00142 | |||
| ∑H (Fraction) | 1.0264 | 1.0027 | A = 6.948; B = -2.545; C = 0.3643; D = -0.01744 | |||
| Cr-1 | 5.0119E-5 | 5.0119E-5 | A = 2.733E-5; B = -1.786E-5; C = -4.387E-6; D = -4.807–7; E = 1.984E-8 | |||
| Cr-H | 0.9999 | 0.9999 | 0.9999 | 0.9999 | A = 1.002; B = -1.142E-3; C = -2.642E-4; D = -2.712–5; E = 1.042E-6 | |
| Cr-H2 | 1.1949E-16 | 1.1949E-17 | A = 1.122E-13; B = -6.392E-14; C = -1.372E-14; D = -1.313E-15; E = 4.729E-17 | |||
| ∑H (Fraction) | 1.008 | 1.008 | A = 1.004; B = 2.197E-3; C = -5.145E-4; D = 5.349–5; E = -2.084E-6 | |||
| Glycolysis | ||||||
| Glc-1 | 6.7608E-7 | 6.7608E-6 | A = -0.00061; B = 0.000522; C = -8.587E-5; D = 4.728E-6 | |||
| Glc-H | 0.9999 | 0.9999 | 0.9999 | 0.9999 | A = 1.001; B = -0.0005051; C = 8.317E-5; D = -4.585E-6 | |
| ∑H (Fraction) | 0.9999 | 0.9999 | A = 1.001; B = -0.0005051; C = 8.317E-5; D = -4.585E-6 | |||
| G1P-1 | 0.4218 | 0.7913 | A = 5.187; B = -4.085; C = 0.876; D = -0.05459 | |||
| G1P-H | 0.5516 | 0.1095 | 0.5189 | 0.0973 | A = -4.924; B = 4.663; C = -0.9997; D = 0.0623 | |
| G1P-H2 | 1.6410E-5 | 3.0790E-7 | A = 0.007625; B = -0.003347; C = 0.0004901; D = -2.394E-5 | |||
| G1P-Mg | 5.9323E-2 | 0.1113 | A = 0.7296; B = -0.5746; C = 0.1232; D = -0.007679 | |||
| ∑H (Fraction) | 0.5189 | 0.0973 | A = -4.917; B = 4.66; C = -0.9992; D = 0.06227 | |||
| G6P-1 | 0.4370 | 0.8859 | A = 16.52; B = -9.52; C = 1.734; D = -0.09897 | |||
| G6P-H | 0.5630 | 0.1141 | 0.5630 | 0.1141 | A = -15.52; B = 9.52; C = -1.734; D = 0.09897 | |
| ∑H (Fraction) | 0.5630 | 0.1141 | A = -15.52; B = 9.52; C = -1.734; D = 0.09897 | |||
| F6P-1 | 0.5630 | 0.9280 | A = -10.98; B = 3.216; C = -0.2088; D = -0.00107 | |||
| F6P-H | 0.4370 | 7.2033E-2 | 0.4370 | 7.2033E-2 | A = 11.98; B = -3.215; C = 0.2087; D = 0.001079 | |
| F6P-H2 | 5.5016E-6 | 9.0684E-8 | A = 0.002784; B = -0.001226; C = 0.0001801; D = -8.821E-6 | |||
| ∑H (Fraction) | 0.4370 | 0.0720 | A = 11.99; B = -3.217; C = 0.209; D = 0.001061 | |||
| F1,6P-4 | 0.10371 | 0.5531 | A = 66.06; B = -31.07; C = 4.814; D = -0.2445 | |||
| F1,6P-H | 0.8136 | 0.3039 | 0.4527 | 0.2414 | A = -104.6; B = 46.72; C = -6.87; D = 0.3337 | |
| F1,6P-H2 | 0.3765 | 2.0081E-2 | A = 27.92; B = -9.98; C = 1.15; D = -0.0419 | |||
| F1,6P-H3 | 7.8669E-5 | 4.1955E-7 | A = 0.04835; B = -0.02141; C = 0.003159; D = -0.0001555 | |||
| F1,6P-H4 | 9.0324E-9 | 4.8171E-12 | A = 9.113E-6; B = -4.094E-6; C = 6.127E-7; D = 3.056E-8 | |||
| F1,6P-Mg | 3.1186E-2 | 0.1663 | A = 19.87; B = -9.343; C = 1.447; D = -0.07353 | |||
| F1,6P-HMg | 3.581E-2 | 1.9096E-2 | A = -8.275; B = 3.695; C = -0.5434; D = 0.0264 | |||
| ∑H (Fraction) | 1.2418 | 0.3007 | A = -56.91; B = 30.39; C = -5.105; D = 0.2759 | |||
| DHP-1 | 0.5505 | 0.9077 | A = -10.7; B = 3.126; C = -0.2012; D = -0.001206 | |||
| DHP-H | 0.4427 | 7.3588E-2 | 0.4372 | 7.2099E-2 | A = 11.94; B = -3.195; C = 0.2057; D = 0.001233 | |
| DHP-Mg | 1.2271E-2 | 2.0234E-2 | A = -0.2386; B = 0.06968; C = -0.004484; D = 2.688E-5 | |||
| ∑H (Fraction) | 0.4373 | 7.210E-2 | A = 11.94; B = -3.195; C = 0.2057; D = 0.001233 | |||
| G3P-1 | 0.2619 | 0.7801 | A = 54.45; B = -26.23; C = 4.155; D = -0.2146 | |||
| G3P-H | 0.7381 | 0.2199 | 0.7381 | 0.2199 | A = -53.45; B = 26.23; C = -4.155; D = 0.2146 | |
| ∑H (Fraction) | 0.7381 | 0.2199 | A = -53.45.; B = 26.23; C = -4.155; D = 0.2146 | |||
| 1,3BPG-1 | 3.0653E-2 | 0.2403 | A = -14.27; B = 7.403; C = -1.288; D = 0.07518 | |||
| 1,3BPG-H | 0.9696 | 0.7597 | 0.9693 | 0.7597 | A = 15.27; B = -7.403; C = 1.288; D = -0.07518 | |
| ∑H (Fraction) | 0.9693 | 0.7597 | A = 15.27; B = -7.403.; C = 1.288; D = -0.07518 | |||
| 3PG-1 | 0.3814 | 0.8605 | A = 30.42; B = -15.81; C = 2.671; D = -0.1452 | |||
| 3PG-H | 0.6186 | 0.1395 | 0.6186 | 0.1395 | A = -29.42; B = 15.81; C = -2.671; D = 0.1452 | |
| ∑H (Fraction) | 0.6186 | 0.1395 | A = -29.42; B = 15.81; C = -2.671; D = 0.1452 | |||
| 2PG-1 | 7.7010E-2 | 0.2509 | A = 19.05; B = -9.065; C = 1.419; D = -0.0725 | |||
| 2PG-H | 0.9091 | 0.5573 | 0.7701 | 0.2509 | A = -55.86; B = 27.06; C = -4.236; D = 0.2164 | |
| 2PG-Mg | 1.3023E-2 | 4.2431E-2 | A = 3.221; B = -1.533; C = 0.24; D = -0.01226 | |||
| 2PG-K | 0.1399 | 0.4557 | A = 34.59; B = -16.46; C = 2.577; D = -0.1317 | |||
| ∑H (Fraction) | 0.7701 | 0.2509 | A = -55.86; B = 27.06; C = -4.236; D = 0.2164 | |||
| PEP-1 | 0.2085 | 0.3603 | A = -2.13; B = 0.2359; C = 0.07674; D = -0.008518 | |||
| PEP-H | 0.6912 | 0.1829 | 0.4667 | 8.0650E-2 | A = 5.792; B = -0.3185; C = -0.2374; D = 0.02377 | |
| PEP-H2 | 1.3153E-3 | 2.2730E-5 | A = 0.6447; B = -0.2836; C = 0.0416; D = -0.002036 | |||
| PEP-Mg | 2.2761E-2 | 3.3933E-2 | A = -0.2326; B = 0.02576; C = 0.008379; D = -0.00093 | |||
| PEP-K | 0.3008 | 0.5197 | A = -3.073; B = 0.3404; C = 0.1107; D = -0.01229 | |||
| ∑H (Fraction) | 0.4693 | 8.070E-2 | A = 7.081.; B = -0.8856; C = -0.1542; D = 0.0197 | |||
| PYR-1 | 0.9923 | 0.9925 | A = 0.9502; B = 0.01817; C = -0.002608; D = 0.0001251 | |||
| PYR-H | 1.8194E-4 | 1.8197E-5 | 1.8057E-4 | 1.8060E-5 | A = 0.0427; B = -0.01832; C = 0.00263; D = -0.0001262 | |
| PYR-Mg | 7.4956E-3 | 7.4968E-3 | A = 0.007175; B = 0.0001384; C = -1.988E-5; D = 9.545E-7 | |||
| ∑H (Fraction) | 1.8057E-4 | 1.8060E-5 | A = 0.0427; B = -0.01832; C = 0.00263; D = -0.0001262 | |||
| La-1 | 0.9897 | 0.9938 | A = -0.08489; B = 0.4629; C = -0.06641; D = 0.003186 | |||
| La-H | 4.6556E-3 | 4.6752E-4 | 4.6292E-3 | 4.6485E-4 | A = 1.085; B = -0.4655; C = 0.06679; D = -0.003204 | |
| La-Mg | 5.6709E-3 | 5.6947E-3 | A = -0.0004883; B = 0.002653; C = -0.0003806; D = 1.826E-5 | |||
| ∑H (Fraction) | 4.6292E-3 | 4.6485E-4 | A = 1.085; B = -0.4655; C = 0.06679; D = -0.003204 | |||
#for most prevalent metabolite-H+ complex
*Binding fraction = A + Bx + Cx2 + Dx3 + Ex4, x = pH