Fig 3. Size differences in allele-specific PCR products.

A) Schematic of PCR amplicons originating from ancestral and derived genomic templates. For both PCR amplicons, gray represents sequence originating from the primers (forward and reverse) and synthesized internal sequence from PCR extension. The primer sequence and synthesized internal sequence within the amplicon are indicated by the bracket. For the derived amplicon, the blue represents the incorporated GC-clamp which originates from the 5’end of the derived MAMA forward primer. The SNP region for each amplicon is represented in green (ancestral) and yellow (derived) and the deliberate antepenultimate mutation is represented as red (ancestral) and light blue (derived). B) Allele-specific PCR products migrate at different rates on a 2% agarose gel due to their size difference as conferred by the GC-clamp.