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. 2017 Dec 21;13(12):e1006745. doi: 10.1371/journal.ppat.1006745

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© 2017 Gurung et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — A) Gating strategy for detection of TGF-beta+ Treg cells are shown. Mononuclear cells were isolated from spleens of 3 weeks-old naïve chicken lines, and were surface stained with anti-CD4-PE, anti-CD25-FITC and anti-TGF-beta-APC mAbs or isotype controls. The 7AAD staining was used for dead cell exclusion. The percentages and mean fluorescent intensity (MFI) of CD4⁺ cells expressing TGF-beta are shown in FACS density plots. The percentages of (B) TGF-beta⁺CD4⁺ T cells, (C) TGF-beta⁺CD4⁺CD25⁺ T cells and (D) CD4⁺CD25⁺ T cells in different chicken lines are shown. The values are representative from five different birds for each group. The mean ± SD value are shown. * indicates a statistically significant difference (P < 0.05); ns, not significant.