(A) Genomic DNA determined by RT-PCR in WT and PiT-2 KO mice. (B) Gross appearance of female mice. (C) Body weight of male and female mice. (D) Representative rendered micro CT scan showing the various bone length measurements taken. Length of (E) vertebral column, (F) femur, and (G) tibia of male mice. Data are presented as mean ± S.E.M (n=5). A two-tailed P-value < 0.05 was considered statistically significant. *P<0.05 versus WT group. #P<0.05 versus female WT group. Micro CT, micro-computed tomography; PiT-2 KO, PiT-2 homozygous knockout; RT-PCR, reverse transcriptase-polymerase chain reaction; WT, wild-type.