Table 1.

Characterization of neuroblastoma cell lines and ED50 of topotecan used as a single agent or in combination with mAb 8B6.

		ED50 of topotecan(µM) a
Cell line	MYCN Amplification	Without mAb 8B6	With mAb 8B6	p value
NXS2	No 53	4.7 ± 0.3	1.2 ± 0.15	< 0.01
IMR5	Yes 54	0.010 ± 0.001	0.005 ± 0.001	< 0.01
LAN1	Yes 55	7.5 ± 1.2	1.9 ± 0.2	< 0.01
LAN5	Yes 56	0.024 ± 0.007	0.005 ± 0.001	< 0.01

^aED₅₀ were calculated using CompuSyn software as described in the Material and Methods Section. Data represent the mean of 3 independent experiments ± SEM.

^bAnti-OAcGD2 mAb 8B6 was combined with topotecan at the concentration of 40 µg/ml.