Figure 4.

siRNA-silencing of A20 and CYLD augments costimulation triggered by CD137 stimulation on human primary CD8 T cells. (A) Schematic representation of the experiments. (B) Human CD8 T cells were electroporated with the indicated siRNAs and underwent stimulation with anti-CD3 plus anti-CD137 mAb. Four days later CD25 and Bcl-xL expression were monitored by immunofluorescence and flow cytometry. Data are shown as mean intensity of fluorescence (mean ± SEM). Downregulation of the corresponding mRNAs was confirmed by qRT-PCR (not shown). A representative experiment out of at least 3 independently performed is shown. Statistical comparisons were performed using students t tests (* p < 0.05, **p < 0.01, ***p < 0.001).