Figure 5.

A20 and CYLD decrease CD137-elicited NF-κB activation in vivo. (A) Mice (6 per group) were hydrodinamically gene transferred to the liver with expression plasmids encoding the NF-κB luciferase reporter construct and the indicated genes (A). 48 hours later mice were dosed intraperitoneally with the anti-CD137 mAb 6B4 (B) or urelumab (C) and bioluminiscence was monitored by light emission from the upper abdomen (photons/sec in B and fold change over baseline in C). (D) Shows images of representative mice in B. Baseline was measured immediately before intraperitoneal mAb injection. Results represent mean ± SEM of an experiment out of at least 4 performed under similar conditions and rendering similar results.