Figure 5.

Treatment with TCR+iIL-12 T cells results in increased plasma levels of inflammatory cytokines. Plasma samples were collected from mice on days 16 and 21 after T cell transfer and were screened for the presence of multiple cytokines via multiplex assay (“ProcartaPlex Mouse Th1/ Th2/ Th9/ Th17/ Th22/ Treg Cytokine Panel (17 plex)” by eBioscience). Concentrations of IL-12, IL-18, IFNγ, IL-2, TNFα and IL-10 on day 16 are presented as mean±SEM (n = 3). Statistically significant differences between treatment groups were calculated with Student's t-test: *p < 0.05; **p < 0.01.