Figure 7.

Mice treated with TCR+iIL-12 T cells show an enhanced number of tumor-infiltrating macrophages. Following T cell treatment, regressing tumors were collected at day 5 after T cell transfer. Tumors were dissociated into a single-cell suspension, stained with antibodies directed against CD3 (T cells), CD68 (macrophages), CD335 (NK cells) or Ly6G (neutrophils), and analyzed by flow cytometry. Figure depicts the mean number for each cell type ±SEM (n = 4). Statistically significant differences between treatment groups were calculated with Student's t-test: *p < 0.05; **p < 0.01.