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. 2017 Sep 21;7(1):e1372080. doi: 10.1080/2162402X.2017.1372080

Figure 2.

Figure 2.

BTN3A isoform expression in pancreatic cell lines. (A) Representative overlays of BTN3A global surface expression (black line) compared to isotype control (grey line) in pancreatic cell lines (n = 5) as assessed by flow cytometry (upper panel) and in PDX-derived cell lines (n = 4) using an anti-BTN3A mAb (lower panel). (B) Transcriptional expression of BTN3A isoforms. Representative expression of the three BTN3A isoforms in pancreatic- (upper panel) and PDX-derived cell lines (lower panel). qRT PCR analyses were performed on total RNA isolated from pancreatic cell lines. Data were normalized using Peptidylpropyl isomerase A (PPIA) as an endogenous control (ΔCt = CtTarget gene – CtPPIA). Results were expressed as mean 2−ΔCt and shown as percentage of total quantified BTN3A isoforms. (C) BTN3A protein expression. Western Blot analysis of total protein extracts of pancreatic cell lines. Extracts were loaded in 10% SDS PAGE gel and membranes were hybridized with anti-BTN3A 20.1 mAb and anti-Grb2 as a loading control.