Figure 5.
The anti-Vγ9Vδ2-TCR specific VHH 6H4 efficiently activates Vγ9Vδ2-T cells harbouring δ2-CDR variations. (A) Indicated JurMa transductants were incubated with 500 nM VHH 6H4 and bound VHH was assessed by flow cytometry. Mean fluorescence intensity (MF) of VHH bound to the cells is depicted. A representative figure of n = 3 experiments is shown. (B) Indicated JurMa transductants were cultured with HeLa cells (negative control, white), NBP-pretreated HeLa cells (positive control, grey) or plate bound (wells coated with 500 nM) VHH 6H4 (black). After 24 hrs, the activation status of the cells was determined by assessing CD69 expression on the cells by flow cytometry. Indicated significant differences are relative to values of δ2-G115WT cells stimulated with HeLa cells. A representative figure of triplicate samples (mean ± SEM) of n = 3 experiments is shown. p-Values were calculated with a one-way ANOVA and Bonferroni's post-hoc test (*** indicates p<0.001). Abbreviations: aminobisphosphonates (NBP).