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ROC analyses for cut-off determination for EV levels pre- and post-NACT and Kaplan-Meier survival analysis regarding 3y-PFS and OS. (A)/(C) By ROC analysis a consistent cut-off value of 2484 ×109/ml for EV levels post-NACT was obtained for 3y-PFS and OS. (B/D) In Kaplan Meier analysis combined with the log rank test, BC patients having EV levels post-NACT above the determined cut-off value had a significantly reduced 3y-PFS and OS.
