Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 27;7(1):e1377872. doi: 10.1080/2162402X.2017.1377872

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

PMC Copyright notice

Figure 5. — Immunological-response assessment in case 2. (A) The raw data of ex vivo IFN-γ ELISPOT assay using PBMCs of case 2. Dextramer assay using PBMCs of case 2 were performed after in vitro peptide stimulation. (B) Ex vivo GPC3-Dextramer staining after vaccination. GPC3-peptide-specific CTL frequency is indicated as the percentage of Dextramer-positive CTLs to CD8-positive cells in the tumor-resected specimen (red circle). (C) Dextramer analysis of the establishment of the GPC3-peptide-specific CTL clones in the tumor-biopsy specimen. (D) IFN-γ ELISPOT assay against SK-Hep-1/vec, SK-Hep-1/hGPC3, and peptide-pulsed T2A24. Effector / target (E / T) ratio = 0.2. (E) Externalized CD107a analysis of the establishment of the GPC3-peptide-specific CTL clones in the tumor-biopsy specimen. T2A24 pulsed with GPC3_298–306 or HIV_583–591 peptide were used as target cells. (F) TNF-α levels in the CTL clone (TIL clone.1) (1.0 × 10⁵ cells/well) after a 24-h co-culture with the indicated target cells (5 × 10⁴ cells/well). Data represent the mean ± standard deviation of triplicate cultures. *p < 0.05.