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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Glia. 2017 May 22;65(8):1264–1277. doi: 10.1002/glia.23160

Figure 2. HO-1 protein loss is associated with immunoproteasome induction in HIV-infected brain.

Figure 2

Expression of immunoproteasome subunits LMP7 (A, B, C) and PA28α (D, E, F) was determined by Western blot in DLPFC samples. Immunoproteasome expression was correlated with (A, D) HO-1 RNA in 64 HIV− and 87 HIV+ samples and with (B, E) HO-1 and (C, F) HO-2 protein in 65 HIV− and 88 HIV+ DLPFC samples. Correlations were determined by Pearson’s correlation with line of best fit determined by linear regression. (G) Immunoproteasome subunit LMP2 was visualized in subcortical white matter by immunohistochemistry (top panels) and immunofluorescence (bottom panels). Scale bar = 200 μm. (H) Immunoproteasome subunits were localized to astrocytes by dual indirect immunofluorescence staining of LMP2 (top panels) or PA28α (bottom panels) with the astrocyte marker GFAP in subcortical white matter of HIVE cases. Scale bar =10 μm.