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. 2017 Aug 24;8(62):104867–104876. doi: 10.18632/oncotarget.20452

Figure 1. miR-200a expression depletes Keap1 to cause Nrf2 accumulation in human osteoblastic cells.

Figure 1

MicroRNA-200a-3p (“miR-200a-3p”) putatively targets the 3′-UTR of Keap1 mRNA (A). Stable OB-6 osteoblastic cells, expressing miR-200a expression vector (“miR-200a-Vec”, two lines, “Line1/2”), non-sense microRNA-control (“miR-C”), or the parental control OB-6 cells (“Ctrl”) were subjected to quantitative real-time PCR (“qRT-PCR”) assay (B and D), Keap1 mRNA 3′-UTR luciferase activity assay (C) and Western blotting assay (E, results were quantified). Relative expression of miR-200a-3p (F), Keap1 mRNA (G), Keap1/Nrf2 protein (H) and Nrf2 mRNA (I) in stable OB-6 cells with miR-200a anti-sense (“antamiR-200a”), antagomiR-control (“antamiR-C”), as well as in parental control cells (“Ctrl”) were shown. Data were expressed as mean ± SD (n=5). * p <0.05 vs. “Ctrl”. Experiments in this figure were repeated three times, and similar results were obtained.