Figure 6. The effect of methotrexate (MTX), 6-mercaptopurine (6-MP) and 5-fluorouracil (5-FLU) on cell cycle progression, when used in combination with apigenin (AP): in two lymphoid leukemia cell lines (Jurkat and CCRF-CEM) and two myeloid leukemia cell lines (THP-1 and KG-1a).

This was analysed by flow cytometry following propidium iodide staining. Cells were treated with MTX, 6-MP or 5-FLU and apigenin alone and in combination for 24 hrs using their lowest-significant doses (LSD) as determined by CellTiter-Glo assay, together with a vehicle control. The percentage of cells in each phase was analysed with FlowJo software using Waston pragmatic model. The data was expressed as medians with ranges (n=4). Statistical significance of combination treatments were determined and compared with the vehicle control and the individual treatments alone. The green asterisk (^*) represents significant increase in cell accumulation in a phase of the cell cycle; whilst the black asterisk (^*) indicates a significant decrease in cell accumulation in a phase of the cell cycle. Statistical significant was set at P≤0.05.