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. 2017 Oct 27;8(62):105238–105250. doi: 10.18632/oncotarget.22164

Figure 1. Expression levels of CTHRC1 in HCC tissue and cell lines.

Figure 1

(A) Western blot analysis of CTHRC1 expression in HCC tissues compared with paired corresponding non-tumorous tissues (T, tumor; N, non-tumor tissues). (B) CTHRC1 mRNA levels were measured in six groups: normal liver (NL, n = 17), liver cirrhosis (LC, n = 21), Edmondson grade I (GI, n = 8), Edmondson grade II (GII, n = 51), Edmondson grade III (GIII, n = 63), and Edmondson grade IV (GIV, n = 6). Bars indicate medians. Mann-Whitney U tests was used for the calculation of P values. The Kruskal-Wallis test was used for overall comparison. *P < 0.05; ***P < 0.001. (C) Overview of CTHRC1-positive HCC tissues (a-c). CTHRC1 immunoreactivity was correlated in part with normal (d) and advancement of Edmondson differentiation (e-h). (D) Western blot analysis of CTHRC1 expression in HCC cell lines. Lysates of HepG2 cells transduced with adenovirus LacZ or CTHRC1 were used as negative or positive controls, respectively. (E) Western blot analysis of secreted CTHRC1 in HCC cell supernatant. Proteins detected by Coomassie Blue staining served as an internal control for protein loading.