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. 2017 Oct 27;8(62):105238–105250. doi: 10.18632/oncotarget.22164

Figure 4. Downstream signaling of CTHRC1.

Figure 4

(A) Adeno-associated virus expressing CTHRC1 or LacZ was transiently transduced into HLK-3 cells at a multiplicity of infection (MOI) of 100-200. Forty-eight hours after infection, overexpression of CTHRC1 in HLK-3 cells was confirmed by western blot analysis. (B) The signaling protein molecules are indicated in the box. The two doublets in the corners are used as internal positive controls. (C) Phosphorylation of Akt was measured by immunoblot after AdCTHRC1 or AdLacZ transduction into HLK-3 and HepG2 cells. The results shown are from three independent experiments. (D) Activation of PI3K, PDK, Akt, and ERK in the stable CTHRC1 transfectants compared to vector control cells. (E) Activation of PI3K, Akt, and ERK in the adeno-associated virus expressing CTHRC1 compared to mock control cells in HLK-3 cells or (F) in HepG2 cells.