Figure 6. TWEAK–NF-κB signaling pathway was significantly inhibited in RDH10 knockdown U87 cells.
(A) IPA showing that TWEAK signaling was one of the most significantly inhibited pathway. (B) qPCR was used to validate the mRNA expression of related genes. (C) Western blotting was used to validate the protein expression levels of related genes. (D) Densitometry showed relative protein expression levels of related genes. (E and F) Gene interaction network of RDH10 and related genes in TWEAK–NF-κB signaling pathway. The results show that NFKBIA is located in the central regulatory position. Data are shown as mean ± SD.