Figure 5. Agrin knockdown and its effect on cell proliferation, migration, and EMT.

(A) The effect of siRNA transfection on agrin expression. Both agrin protein and cDNA levels were downregulated in cells transfected with siRNA versus controls. (B) Absorbance curves showing cell proliferation ability of agrin knockdown (siRNA) cells versus controls. The agrin knockdown cells showed a markedly lower ability to proliferate. (C and D) Transwell migration (C) and wound healing (D) assays showing the metastatic and invasive ability of the cells. Both the invasive and metastatic abilities of agrin knockdown cells were impaired in comparison with the controls. (E) Western blot showing the reversal of the EMT process in agrin knockdown cells. E-cadherin was upregulated while vimentin was downregulated in the knockdown sample.