Figure 2. Knockdown of p22^phox in FLT3-ITD expressing MV4-11 cells had no effect on NOX4 67 kDa and NOX4D 28 kDa protein expression. NOX4D 28 kDa is glycosylated in FLT3-ITD expressing AML.

(A) Western blot analysis of p22^phox, NOX4 67 kDa and NOX4D 28 kDa protein levels in MV4-11 whole cell lysates at 24 h following p22^phox siRNA transfection. β-actin was used as a loading control. (B) Western blot analysis of NOX4 67 kDa and NOX4D 28 kDa protein expression in whole cell lysates following treatment with tunicamycin (5 μg/ml) (i) and brefeldin A (10 μg/ml) (ii) overnight. (Asterisks indicate a shift in protein molecular weight). β-actin and total protein were used as loading controls. Blots are representative of three independent experiments.