Figure 3. Exogenous over-expression of SphK2 promotes OS cell growth.

mRNA (A) and protein (B) expression of SphK2 in stable U2OS cells expressing SphK2 cDNA (two lines, “SphK2-cDNA-L1/L2”) or the empty vector (pSuper-EGFP-puro-Flag), as well as in parental control cells (“Par”), were shown; Cells were also subjected to cell counting assay (C) to test cell growth. Data were shown as mean (n=5) ± standard deviation (SD). ^*p<0.05 vs. “Par” cells. Experiments in this figure were repeated three times, and similar results were obtained.