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. 2017 Nov 11;8(62):105832–105847. doi: 10.18632/oncotarget.22405

Figure 1. Workflow and phenotypes of animal models.

Figure 1

(A) Workflow of experiments. a. Construction of animal models; b. examination of phenotype; c. PTC isolation; d. libraries for preparation of RNA-sequencing; e. next-generation sequencing; f. bio-information analysis. (B) Fasting blood sugar over time. Data are represented as mean ±SEM. *p < 0.05 by t-test versus control at 0 week. #p < 0.05 by t-test versus control at the same week. n = 6 for each group. (C) ACR over time. Data are represented as mean ±SEM. *p < 0.05 by t-test versus control at 0 week. #p < 0.05 by t-test versus control at the same week. n = 6 for each group. (D) Left: PAS-stained sections in each group. Images display kidney sections of each group at 200× magnification. White arrow indicates normal tubule, and black arrow indicates injured tubule. Views with high magnification are shown at lower left. Time course are labeled as a, b, c, and d; letters correspond to 0, 2, 4, and 8 weeks. Right: Tubule injuries were evaluated for widened lumen, atrophy, or thickened basement membranes. Tubular injuries are indicated with arrows. Bar graph shows TDI for each group. Data are represented as mean ±SEM. *p < 0.05 by t-test versus control in 0 week. n = 6 for each group. (E) Left: Masson-trichrome-stained kidney sections in each group. Images display kidney sections of each group at 200× magnification. White arrow indicates normal tubule, and black arrow indicates injured tubule. Views with high magnification are shown at lower left. Time courses are labeled as a, b, c, and d, corresponding to 0, 2, 4, and 8 weeks. Right: Bar graph shows relative interstitial volume for each group. Data are represented as mean ±SEM. n = 6 for each group.