Figure 5. Effect of andrographolide on MAPK pathway in GBM8401 cells.

GBM8401 cells were treated with andrographolide (0-40 μM) for 24 h and then the cell lysates were subjected to SDS-PAGE followed by western blots with (A) anti-ERK1/2, anti-JNK, anti-p38, (C) anti-c-Raf and anti-MEK antibodies as described in Materials and Methods. (B, D) Quantitative results the phosphorylation levels of ERK1/2, JNK, p38, c-Raf and MEK. ^*Significantly different, p < 0.05, compared with the vehicle group.