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. 2017 Nov 11;8(62):105873–105881. doi: 10.18632/oncotarget.22411

Figure 3. Effects of a forced reversal of the miR-20a-5p or NPAS2 levels on the radio-resistance of CNE-1 and CNE-2 cells.

Figure 3

MiR-20a-5p mimic (5PM)-transfected CNE-2 increases NC cells survival fraction versus the negative control (NC) cells (A). NPAS2 protein level (Western blot analysis) and mRNA determined by qRT-PCR in the si-NPAS2-transfected versus the NC-transfected CNE-2 cells treated with a 4-Gy dose of radiation (B and C). Si-NPAS2-transfected CNE-2 increases NC cells survival fraction versus the negative control (NC) cells (D). MiR-20a-5p antagomiR (5PA)-transfected CNE-1 decreases NC cells survival fraction versus the negative control (NC) cells (E). The surviving fraction was calculated using the multitarget single-hit model: Y=1-(1-exp(−k*x))^N. The data are presented as the mean±standard deviation of results from 3 independent experiments, and two way anova was used to calculate statistical significance.