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. 2017 Nov 18;8(62):105923–105935. doi: 10.18632/oncotarget.22510

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Copyright: © 2017 Jiao et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A) Schematic structure of human USP18 and its deletion mutants used in this study, UCH indicate the UCH (ubiquitin C-terminal hydrolase) domains. The IRS4 binding domains is indicated in the right column. (B) Co-IP assay of the interaction between USP18 mutants and Myc-IRS4. 293T cells co-expressing Flag-USP18 mutants and Myc-IRS4 were lysed and immunoprecipitated with an anti-Flag antibody. Immunoprecipitates and cell lysates were analyzed by immunoblotting with anti-Myc or anti-Flag antibody. (C) Co-IP assay of the interaction between USP18 mutants and endogenous IRS4. Cell protein lysates were then immunoprecipitated with an anti-Flag antibody followed by immunoblotting with anti-IRS4(upper) or anti-Flag antibody (lower). HC, heavy chain; LC, light chain.