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. 2017 Nov 20;8(62):106026–106037. doi: 10.18632/oncotarget.22523

Figure 4. β-Catenin is the crucial target of RTL1 in melanoma.

Figure 4

(A) QPCR assay detecting the overexpression and knockdown efficiency of β-Catenin. Compared to the -DOX group, ** p<0.01; (B) western blot assay detecting the overexpression and knockdown efficiency of β-Catenin; the protein level had been analyzed by Densitometry, compared with the control group (FUGW and Tet-on shRNA without DOX), * p<0.05. (C) The TOP flash and FOP flash relative luciferase activity assays were used to detect the activity of Wnt signalling in the RTL1-overexpressing cell line with β-Catenin knockdown, which was also performed in the RTL1-knockdown cell line with β-Catenin overexpression. Compared to FUGW or Scramble, * p<0.05 and ** p<0.01. Compared to cells with RTL1 overexpression or knockdown, ## p<0.01; (D) the cell proliferation assay for β-Catenin knockdown (left panel) or overexpression (right panel) in RTL1-overexpressing or RTL1-knockdown cells; (E) cell cycle assay for β-Catenin knockdown (left panel) or overexpression (right panel) in RTL1-overexpressing or RTL1-knockdown cells; (F) QPCR assay for the expression of cell cycle related genes of β-Catenin knockdown (left panel) or overexpression (right panel) in RTL1-overexpressing or RTL1-knockdown cells. Compared to FUGW or Scramble, * p<0.05 and ** p<0.01. Compared to Tet-on β-Catenin knockdown or overexpression without DOX, # p<0.05 and ## p<0.01.