Figure 6. Modulation of proximal tubular induction of Snail1, P-cadherin, ILK1, GSK-3β and β-catenin by oral treatment of eucalyptol.
The db/db mice were orally administrated with 10 mg/kg eucalyptol for 8 weeks. Immunohistochemical staining was performed to observe proximal tubular Snail1 in db/db mice (A). Magnification: 200-fold. Tissue extracts were subject to 8-12% SDS-PAGE and Western blot analysis with a primary antibody against P-cadherin, ILK1, GSK-3β or β-catenin (B). β-Actin was used as internal control. The bar graphs (mean ± SEM, n=3) in the right panels represent quantitative desitometric results. Values not sharing a common letter are significantly different at P<0.05.