Table 4. Primer sequences for miR-34a, U6 and miRNA target genes.
Gene | Foward primer( 5′→ 3′) | Reverse primer( 5′→3′) |
---|---|---|
miR-34a | GGGTGGCAGTGTCTTAGC | CAGTGCGTGTCGTGGAGT |
U6 | GTGCTCGCTTCGGCAGCA | CAAAATATGGAACGCTTC |
P53 | TCTTCCTACAGTACTCCCCT | GCTTGCTTACCTCGCTTAGT |
Bcl-2 | ACACCCCAATTCTTCCTGCCC | AATCCTCCCCCAGTTCACCC |
Top-2a | GCGAGTGTGCTGGTCACTAA | ACAATTGGCCGCTAAACTTG |
CCND1 | GTCTTCCCGCTGGCCATGAACTAC | GGAAGCGTGTGAGGCGGTAGTAGG |
Notch1 | CACTGTGGGCGGGTCC | GTTGTATTGGTTCGGCACCAT |
β-actin | TCATGAAGTGTGACGTGGACATC | CAGGAGGAGCAATGATCTTGATCT |