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. 2017 Nov 18;8(63):106565–106576. doi: 10.18632/oncotarget.22497

Figure 2. Stable knockdown of TAB3 reduced Survivin expression and inhibited CRC invasion and metastasis in vitro and in vivo.

Figure 2

(A) qRT-PCR and Western blot analyses of TAB3 and Survivin expression in the indicated CRC cell lines. (B) Western blot analyses were used to detect TAB3 and Survivin expression in SW480 and LOVO cells stably transfected with shNC, shTAB3#1 and shTAB3#2. (C) Wound healing assay. Wound closure was delayed in stable TAB3-knockdown cells compared with the shNC control at both the 24- and 36-h time points. Magnification, ×100. (D) Transwell migration assays of SW480 and LOVO cells with TAB3 expression stably inhibited (**p < 0.01). Magnification, ×100. (E) Transwell invasion assays of SW480 and LOVO cells treated with shNC, shTAB3#1 and shTAB3#2. Magnification, ×100. (F) Representative H&E staining of livers from the SW480-shNC and SW480-shTAB3 #1 groups. Magnification: a and b, ×100; c and d, ×400. (G) (n = 8; **P < 0.01).