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. 2017 Nov 18;8(63):106565–106576. doi: 10.18632/oncotarget.22497

Figure 4. TAB3 regulates Survivin expression through the NF-κB pathway.

Figure 4

(A) The protein expression level of TAB3, IKBa, p-IKBa, P65, p-P65 and Survivin were assessed by Western blotting in TAB3-silenced SW480 cells. (B) Luciferase analysis was used to determine the NF-κB activity in TAB3-knockdown SW480 cells ( **P < 0.01). (C) Western blot analysis showing the levels of TAB3 silencing and activation of the NF-κB pathway (treatment with 10 ng/ml tumor necrosis factor) and their effects on p-P65, Survivin, c-Myc, and MMP-9 in SW480 cells. (D) Activation of the NF-κB pathway rescued cell migration and invasion in SW480-shTAB3 cells (**P < 0.01). (E) Protein expression levels of TAB3, IKBa, p-IKBa, P65, p-P65 and Survivin in HCT116 cells transfected with pcDNA3.1(+)-vector and pcDNA3.1(+)-TAB3. (F) Luciferase analysis was performed using HCT116 cells transfected with the pcDNA3.1(+)-vector or pcDNA3.1(+)-TAB3 plasmid (**P < 0.01). (G) Western blot analysis showing the levels of TAB3 overexpression and NF-κB signaling inhibition (treatment with 10 ng/ml Caffeic Acid Phenethyl Ester) and their effects on p-P65, Survivin, c-Myc and MMP-9 in HCT116 cells.(H.) Blockade of the NF-κB pathway enhanced migration and invasion in HCT116-pcDNA3.1(+)-TAB3 cells (**P < 0.01).