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. 2017 Nov 18;8(63):106598–106607. doi: 10.18632/oncotarget.22503

Figure 2. PCR-RFLP analysis of the SLC10A1 variant c.263T>C (p.I88T) in the two families.

Figure 2

The schematic diagram of the PCR-RFLP procedure was illustrated in (A). Electrophoresis of the PCR-RFLP products (B) demonstrated that patient 1 and her mother were both carriers of c.263T>C, harboring two additional bands of 89 bp and 180 bp arising from Ddel digestion of the mutated PCR products. Her father had the same DNA bands of 453bp and 269bp as in the healthy control. Similarly in (C), patient 2 and his father were both carriers of c.263T>C, while the mother was negative for this variant.