Table 2. Primers for amplification and sequencing analysis and the PCR conditions.
| Targets | NTCP primer sequences(5′ to 3′) | AT (°C) | Polymerase | Products (bp) |
|---|---|---|---|---|
| Promotor▲ | Forward:CACAGTAGGAGGTGGAAGGATTTTG | 58 | LA-Taq | 2117 |
| Reverse: CTTGCTGGATGCCTTCTTTAATC | ||||
| Exon 1 | Forward: GAAACTAAGGAATCAAGAGCGGAGC | 58 | Taq | 1248 |
| Reverse: CAGGAATTTGAGGTGCTCATTTGG | ||||
| Exon 2 | Forward: CCACTTACTACCTTGTGCGACTTTG | 58 | Taq | 991 |
| Reverse: TGGAATTGGATCTTGTTTCTCTCG | ||||
| Exon 3 | Forward: CACACCTGTAATCCCAGCACTTTGG | 58 | Taq | 983 |
| Reverse: GTGTTTGGATACCTTTGGTGTCTG | ||||
| Exon 4 | Forward: CACTTTCCTGGCAATATGTTCAGATG | 58 | Taq | 628 |
| Reverse: GATGGAAGTAGTCTTGGATCTTTAATG | ||||
| Exon 5 | Forward: CGAAGTTAGAAGTGAAGTGATGATGAAG | 58 | Taq | 1432 |
| Reverse: CTGTGTTTCTCGTTTTGGTGTTGG |
AT, annealing temperature; bp, base pairs.▲For the sequencing of the promotor region, an additional primer was used with the nucleotide sequence 5ʹ-CCTAGGATAACCTCACACACTAG-3ʹ.