Table 1. Levels of estrogen and its metabolites in lung tissue*.
| NORMAL | All (pg/g tissue) | Never Smokers (pg/g tissue) | Current Smokers (pg/g tissue) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| W n = 18 |
M n = 17 |
P W vs. M |
FDR | W n = 9 |
M n = 7 |
P W vs. M |
FDR | W n = 9 |
M n = 10 |
P W vs. M |
FDR | |
| E1 | 8.48 | 6.10 | 0.010 | 0.013 | 6.85 | 5.47 | 0.091 | 0.131 | 10.32 | 6.46 | 0.035 | 0.038 |
| E2 | 15.12 | 8.98 | 4.3x10-8 | 1.9x10-7 | 13.11 | 8.73 | 1.8x10-4 | 0.001 | 18.19 | 9.42 | 8.6x10-5 | 1.9x10-4 |
| E3 | 3.53 | 2.57 | 0.003 | 0.004 | 3.02 | 2.14 | 0.210 | 0.249 | 4.93 | 2.67 | 0.003 | 0.004 |
| 2OHE1 | 2.69 | 1.86 | 3.2x10-6 | 6.9x10-6 | 2.32 | 1.69 | 0.003 | 0.007 | 2.97 | 1.98 | 8.7x10-5 | 1.9x10-4 |
| 2OHE2 | 1.28 | 0.71 | 8.6x10-8 | 2.8x10-7 | 1.04 | 0.72 | 0.002 | 0.007 | 2.16 | 0.68 | 4.3x10-5 | 1.4x10-4 |
| 4OHE1 | 0.39 | 0.21 | 1.8x10-5 | 3.3x10-5 | 0.30 | 0.20 | 0.031 | 0.058 | 0.45 | 0.21 | 2.2x10-5 | 1.4x10-4 |
| 4OHE2 | 0.16 | 0.10 | 0.003 | 0.004 | 0.12 | 0.10 | 0.457 | 0.457 | 0.35 | 0.10 | 4.3x10-5 | 1.4x10-4 |
| 2OME1 | 1.47 | 0.51 | 3.1x10-9 | 4.0x10-8 | 1.38 | 0.51 | 3.5x10-4 | 0.002 | 1.55 | 0.52 | 4.3x10-5 | 1.4x10-4 |
| 2OME2 | 0.88 | 0.26 | 8.4x10-9 | 5.4x10-8 | 0.85 | 0.25 | 1.8 x10-4 | 0.001 | 0.92 | 0.30 | 1.5x10-4 | 2.8x10-4 |
| 2OHEs/Total | 0.11 | 0.12 | 0.883 | 0.883 | 0.10 | 0.12 | 0.351 | 0.380 | 0.12 | 0.12 | 0.356 | 0.356 |
| 4OHEs/Total | 0.02 | 0.01 | 0.732 | 0.793 | 0.01 | 0.01 | 0.042 | 0.067 | 0.02 | 0.01 | 0.004 | 0.005 |
| 2OMEs/Total | 0.07 | 0.04 | 7.0x10-7 | 1.8x10-6 | 0.06 | 0.04 | 0.003 | 0.007 | 0.09 | 0.04 | 4.1x10-4 | 6.7x10-4 |
| 4OHEs/2OHEs | 0.13 | 0.12 | 0.503 | 0.595 | 0.11 | 0.12 | 0.142 | 0.184 | 0.17 | 0.12 | 0.013 | 0.016 |
| TUMOR | W n = 17 |
M n = 15 |
P W vs. M |
FDR | W n = 8 |
M n = 5 |
P W vs. M |
FDR | W n = 9 |
M n = 10 |
P W vs. M |
FDR |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E1 | 9.79 | 5.84 | 4.9x10-7 | 1.1x10-6 | 8.77 | 5.20 | 0.002 | 0.003 | 12.02 | 6.79 | 2.2x10-5 | 7.0x10-5 |
| E2 | 20.86 | 9.18 | 1.4 x10-8 | 6.3x10-8 | 18.73 | 8.96 | 0.002 | 0.003 | 24.36 | 10.15 | 2.2x10-5 | 7.0x10-5 |
| E3 | 4.87 | 2.88 | 1.8x10-6 | 2.9x10-6 | 4.22 | 2.26 | 0.011 | 0.016 | 5.18 | 2.90 | 1.5x10-4 | 2.2x10-4 |
| 2OHE1 | 4.10 | 1.96 | 4.9x10-7 | 1.1x10-6 | 3.43 | 1.92 | 0.002 | 0.003 | 4.76 | 2.09 | 4.3x10-5 | 8.0x10-5 |
| 2OHE2 | 1.82 | 0.76 | 7.1x10-9 | 4.6x10-8 | 1.23 | 0.76 | 0.002 | 0.003 | 2.47 | 0.74 | 2.2x10-5 | 7.0x10-5 |
| 4OHE1 | 0.70 | 0.23 | 3.2x10-6 | 4.7x10-6 | 0.69 | 0.23 | 0.011 | 0.016 | 0.70 | 0.24 | 8.7x10-5 | 1.4x10-4 |
| 4OHE2 | 0.31 | 0.12 | 9.5x10-6 | 1.2x10-5 | 0.17 | 0.13 | 0.065 | 0.085 | 0.42 | 0.12 | 2.2x10-5 | 7.0x10-5 |
| 2OME1 | 1.73 | 0.62 | 7.1x10-9 | 4.6x10-8 | 1.73 | 0.49 | 0.002 | 0.003 | 1.73 | 0.63 | 4.3x10-5 | 8.0x10-5 |
| 2OME2 | 1.13 | 0.32 | 2.5x10-8 | 8.0x10-8 | 1.37 | 0.27 | 0.002 | 0.003 | 1.13 | 0.34 | 4.3x10-5 | 8.0x10-5 |
| 2OHEs/Total | 0.12 | 0.12 | 0.411 | 0.411 | 0.12 | 0.12 | 0.222 | 0.262 | 0.11 | 0.11 | 1.000 | 1.000 |
| 4OHEs/Total | 0.02 | 0.02 | 0.261 | 0.283 | 0.02 | 0.02 | 0.943 | 0.943 | 0.02 | 0.02 | 0.095 | 0.103 |
| 2OMEs/Total | 0.06 | 0.04 | 1.8x10-6 | 2.9x10-6 | 0.07 | 0.04 | 0.006 | 0.012 | 0.06 | 0.04 | 0.001 | 0.001 |
| 4OHEs/2OHEs | 0.14 | 0.13 | 0.165 | 0.194 | 0.14 | 0.13 | 0.943 | 0.943 | 0.21 | 0.13 | 0.028 | 0.033 |
*Values represent the medians. Estrogen and its metabolites in lung tissue from NSCLC patients were analyzed by LC-MS2. Statistical comparisons were based on the Wilcoxon rank sum test, with P ≤ 0.05 considered significant (underlined). Bold highlight denotes where both the P values and FDR are ≤ 0.05. W: women; M: men.