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. 2017 Nov 6;8(63):106888–106900. doi: 10.18632/oncotarget.22455

Figure 1. Mucinous appendix cancers/PMP demonstrate COX-2 over-expression.

Figure 1

Explant tissue from six mucinous appendix neoplasms/PMP patients demonstrated significantly higher levels of COX-2 protein expression (A) and mRNA expression (B) than non-mucinous appendix cancers. Representative slides from six separate tumor explants samples are shown. Protein expression in explant tissue was measured by IF staining, slides were stained with COX-2 antibody (green IF), SYTOX Orange was used to stain nucleic acid (blue IF), confocal images were randomly taken of 10 different fields (X 63 magnification) and analyzed using Image-pro Premier Software to quantify the average intensity of COX-2 protein expression. Commercially available primers and probe specific for MUC2 and GAPDH cDNA were used for real-time PCR assay; relative amounts of MUC2 mRNA were determined after normalization of mucin transcripts to that of GAPDH. (IF: immunofluorescence).