Table 3. Univariate and multivariate analysis of associations with overall survival of CRC patients (Cox proportional hazards regression model).
| Cohort 1: protein analysisa | Univariate analysis | Multivariate analysis | ||||
|---|---|---|---|---|---|---|
| Variables | HR | 95% CI | P value | HR | 95% CI | P value |
| Age (≥67 vs. <67 years) | 1.0221 | 0.5392–1.9513 | 0.9464 | - | - | - |
| Sex (female vs. male) | 1.1388 | 0.5899–2.1703 | 0.6940 | - | - | - |
| Tumor size (≥41 vs. <41 mm) | 2.1199 | 0.2450–0.8953 | 0.0217b | 1.2909 | 0.5778–2.9105 | 0.5320 |
| T classification (T3,4 vs. Tis,1,2) | 4.2072 | 1.7953–12.3012 | 0.0004 | 3.5129 | 1.2079–12.7563 | 0.0198 |
| Lymph node metastasis | 3.0386 | 1.5791–6.1326 | 0.0008 | 1.8401 | 0.9048–3.8839 | 0.0927 |
| Distant metastasis | 5.0544 | 2.3611–10.1594 | 0.0001 | 5.5591 | 2.2504–13.6501 | 0.0003 |
| Pathology (poor or mucinous vs. mod/well differentiated) | 4.7735 | 1.9072–10.4208 | 0.0018 | 2.962 | 1.1080–7.0463 | 0.0319 |
| Lymphatic invasion (present vs. absent) | 1.7639 | 0.7896–4.6936 | 0.1765 | - | - | - |
| Venous invasion (present vs. absent) | 2.8751 | 1.5170–5.5277 | 0.0013 | 1.2484 | 0.6071–2.6074 | 0.5470 |
| Ezrin expression (≥5 vs. <5)c | 2.7459 | 1.2651–5.4878 | 0.0124 | 2.3125 | 1.0214–4.8337 | 0.0447 |
| Cohort 2: mRNA analysisd | Univariate analysis | Multivariate analysis | ||||
|---|---|---|---|---|---|---|
| Variables | HR | 95% CI | P value | HR | 95% CI | P value |
| Age (≥67 vs. <67 years) | 2.7073 | 1.5141–5.0460 | 0.0007 | 2.0244 | 0.9946–4.2722 | 0.0518 |
| Sex (female vs. male) | 1.0514 | 0.5306–1.6711 | 0.8629 | - | - | - |
| Tumor size (≥45 vs. <45 mm) | 2.3455 | 1.3196–4.3201 | 0.0035 | 1.3162 | 0.6415–2.6436 | 0.4465 |
| T classification (T3,4 vs. Tis,1,2) | 6.3684 | 0.0963–10.3835 | <0.0001 | 4.5510 | 2.1800–5.1080 | 0.0001 |
| Lymph node metastasis | 3.4421 | 1.9323–6.3528 | <0.0001 | 1.4954 | 0.7406–3.0657 | 0.2611 |
| Distant metastasis | 9.6295 | 5.4338–17.0310 | <0.0001 | 3.8089 | 1.7901–7.8652 | 0.0008 |
| Pathology (poor or mucinous vs. mod/well differentiated) | 1.9778 | 0.8594–3.9946 | 0.1027 | - | - | - |
| Lymphatic invasion (present vs. absent) | 7.0374 | 1.5422–124.5554 | 0.0062 | 1.7671 | 0.3181–33.2918 | 0.5694 |
| Venous invasion (present vs. absent) | 3.1039 | 1.3538–8.9684 | 0.0054 | 1.2428 | 0.1962–4.3652 | 0.7784 |
| Ezrin expression (≥0.58 vs. <0.58)c | 2.2183 | 1.1032–4.3253 | 0.0262 | 1.6608 | 0.7984–3.3463 | 0.1702 |
aFor Cohort 1 protein analysis, the average patient age and tumor size was 66.7 years and 40.8 mm, respectively.
bStatistically significant associations are shown in bold (P < 0.05).
cThe cut-off values for high/low Ezrin expression were 5.0 for protein and 0.58 for mRNA.
dFor Cohort 2 mRNA analysis, the average patient age and tumor size was 67.3 years and 44.6 mm, respectively.