Figure 1. ALS decreases the viability and clonogenic capacity of melanoma cells.

(A) Chemical structure of ALS; (B) MTT assays of A375, skmel-5, and primary melanoma cell viability in response to treatment with ALS at concentrations ranging from 0.01 to 100 μM for 24 hours. IC₅₀ values are shown; (C-E) Clonogenic capacity of A375, skmel-5, and primary melanoma cells as measured by colony formation assays following treatment with ALS at concentrations ranging from 0.01 to 100 μM for 24 hours. Data are expressed as the means ± SD. All experiments were repeated at least three times. (^*p < 0.05, ^**p < 0.01, ^***p < 0.001).