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. 2017 Nov 9;8(63):107134–107148. doi: 10.18632/oncotarget.22359

Figure 2. Manipulation of miR-1271 expression alters letrozole-sensitivity in BCa cells.

Figure 2

(A) RT-qPCR analysis of miR-1271 level in MCF7 cells transfected with anti-miR-1271 or empty control vector (Anti-NC). The untreated MCF7 cells, MCF7 cells transfected with anti-NC and MCF7 cell transfected with anti-miR-1271 in (A) were treated with 10-5 M of letrozole for 9 days, followed by MTT assay (B) and apoptotic ELISA (C). (D) Each mouse received s.c. injections at one site on each flank with 0.1 mL of suspension of transfected MCF7 cells (2×107 cells/mL). Mice were then injected s.c. daily for 32 days with vehicle, androstenedione (Δ4A, 100 μg/d), or androstenedione plus letrozole (10 μg/d) from the day of inoculation. Tumor volumes were measured every 3 days. (E) Tumor xenografts experiment was carried out as described in (D) except that after inoculation mice were treated with vehicle or androstenedione. (F) RT-qPCR analysis of miR-1271 level in MCF7/LR cells transfected with miR-1271 mimic or empty control vector (NC). Transfected MCF7/LR cells in (F) were treated with 10-5 M of letrozole for 9 days, followed by MTT assay (G) and apoptotic ELISA (H). (I) Each mouse received s.c. injections at one site on each flank with 0.1 mL of suspension of transfected MCF7/LR cells (2×107 cells/mL). Mice were then injected s.c. daily for 32 days with vehicle, androstenedione (Δ4A, 100 μg/d), or androstenedione plus letrozole (10 μg/d) from the day of inoculation. Tumor volumes were measured every 3 days. (J) Tumor xenografts experiment was carried out as described in (H) except that after inoculation mice were treated with vehicle or androstenedione.