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. 2017 Nov 9;8(63):107134–107148. doi: 10.18632/oncotarget.22359

Figure 6. DDIT3 is the main downstream effector of miR-1271 signaling.

Figure 6

(A) Effect of DDIT3 knockdown on miR-1271 deficiency-impaired ERα expression was evaluated using immunoblotting. (B) Effect of DDIT3 knockdown on miR-1271 deficiency-impaired ERα pathway was evaluated using RT-qPCR. Different superscript letters denote groups that are statistically different (P<0.05). (C) Effect of DDIT3 knockdown on miR-1271 deficiency-impaired letrozole-sensitivity was determined using apoptotic ELISA. (D) We established the MCF7 cells stably deficient of DDIT3 expression according to a previously reported protocol. Subsequently, cells were subjected to tumor xenografts experiment to reveal the effect of DDIT3 knock-down on tumor growth in vivo. (E) Working model in the current study.