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. 2017 Nov 9;8(63):107157–107166. doi: 10.18632/oncotarget.22362

Figure 4. IGF-IR and IRS1 were two key downstream targets of miR-143 to inhibit VEGF transcriptional activation.

Figure 4

(A) PC-3 cells were treated with docetaxel at different concentration and cultured for 48 h, the transcriptional activation of VEGF was determined using luciferase assay. (B) PC-3 cells were treated with IGF-I at 200 ng/ml for 16 h and subjected to VEGF luciferase assay as above. (C) PC-3 cells stably expressing miR-143 or miR-NC were co-transfected with VEGF reporter, pRL-TK vector plasmid without or with IGF-IR or IRS1 cDNA plasmid at 50 ng (1/4 dose) or 200 ng (1 dose, without indication). The relative luciferase activities of VEGF reporter were assayed and calculated by the ratios of firefly/Renilla luciferase activities, which were normalized to that of the control. Data represent mean±SD. * indicates significant difference at P<0.05; ** indicates significant difference at P<0.01.